US6180341B1

In vitro scanning saturation mutagenesis of proteins

University of Texas System

Abstract

The present invention combines PCR(TM) mutagenesis with in vitro transcription/translation and ELISA for the rapid generation and characterization of protein mutants. The PCR(TM) products are used directly as the template for the in vitro transcription/translation reactions and because no cloning …

University: University of Texas System
Assignee: Board Of Regents, The Universiry Of Texas System
Inventor: Brent L. Iverson
Priority date: May 01, 1997
Filing date: April 30, 1998
Grant date: January 30, 2001
Publication date: January 30, 2001
Language: en
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